BurkDiff: A real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei

Bowers, Jolene R. and Engelthaler, David M. and Ginther, Jennifer L and Pearson, Talima and Peacock, Sharon J. and Tuanyok, Apichai and Wagner, David M. and Currie, Bart J. and Keim, Paul S. (2010) BurkDiff: A real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei. PLoS ONE, 5 (11). e15413. ISSN 1932-6203

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Abstract

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.

Item Type:	Article
ID number or DOI:	10.1371/journal.pone.0015413
Keywords:	Burkholderia mallei; Melioidosis; glanders;
Subjects:	Q Science > QR Microbiology
NAU Depositing Author Academic Status:	Faculty/Staff
Department/Unit:	College of Engineering, Forestry, and Natural Science > Biological Sciences
Date Deposited:	16 Oct 2015 18:00
URI:	http://openknowledge.nau.edu/id/eprint/1738

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