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In vitro selection and characterization of Bacillus anthracis mutants with high-level resistance to ciprofloxacin

Price, Lance B. and Vogler, Amy and Pearson, Talima and Busch, Joseph D. and Schupp, James M. and Keim, Paul (2003) In vitro selection and characterization of Bacillus anthracis mutants with high-level resistance to ciprofloxacin. Antimicrobial Agents and Chemotherapy, 47 (7). pp. 2362-2365. ISSN 1098-6596

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Publisher’s or external URL: http://dx.doi.org/10.1128/aac.47.7.2362-2365.2003

Abstract

Mutants of attenuated Bacillus anthracis with high-level ciprofloxacin resistance were isolated using a three-step in vitro selection. Ciprofloxacin MICs were 0.5 μg/ml for first-step mutants, which had one of two gyrA quinolone resistance-determining region (QRDR) mutations. Ciprofloxacin MICs were 8 and 16 μg/ml for second-step mutants, which had one of three parC QRDR mutations. Ciprofloxacin MICs for third-step mutants were 32 and 64 μg/ml. Mutants for which MICs were 64 μg/ml had one of two additional mutations within the gyrA QRDR or one of two mutations within the gyrB QRDR. Mutants for which MICs were 32 μg/ml had no additional target modifications but showed evidence of enhanced ciprofloxacin efflux.

Item Type: Article
ID number or DOI: 10.1128/AAC.47.7.2362-2365.2003
Keywords: Anti-infective agents; Bacillus anthracis; Bacterial Proteins; Carrier proteins; Ciprofloxacin; dna gyrase; Drug Resistance, Bacterial; fluoroquinolone resistance; genetic testing; gyrA gene; gyrB gene; In Vitro Techniques; Membrane Transport Proteins; Microbial Sensitivity Tests; multidrug efflux transporter; Mutation, Missense; mutations; nora; staphylococcus-aureus; streptococcus-pneumoniae; subtilis; susceptibilities; topoisomerase-iv
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
NAU Depositing Author Academic Status: Faculty/Staff
Department/Unit: Research Centers > Center for Microbial Genetics and Genomics
Date Deposited: 30 Sep 2015 05:39
URI: http://openknowledge.nau.edu/id/eprint/341

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