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Molecular analysis of rifampin resistance in Bacillus anthracis and Bacillus cereus

Vogler, Amy J. and Busch, Joseph D. and Percy-Fine, Stephanie and Tipton-Hunton, Christine and Smith, Kimothy L. and Keim, Paul S (2002) Molecular analysis of rifampin resistance in Bacillus anthracis and Bacillus cereus. Antimicrobial Agents and Chemotherapy, 46 (2). pp. 511-513. ISSN 1098-6596


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Publisher’s or external URL: http://dx.doi.org/10.1128/aac.46.2.511-513.2002


Rifampin-resistant mutants were selected from UV-light-treated Bacillus cereus (20 mutants) and attenuated B. anthracis (23 mutants). In addition, spontaneous rifampin-resistant mutants were also isolated in attenuated B. anthracis (22 mutants). The rifampin resistance clusters of the rpoB gene were sequenced for all 65 mutants. Mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. The spontaneous rate of resistance was estimated at 1.57 × 10−9 mutations/generation by a Luria-Delbrück fluctuation test.

Item Type: Article
ID number or DOI: 10.1128/AAC.46.2.511-513.2002
Keywords: amino acid sequence; Bacillus anthracis; Bacillus cereus; Drug Resistance, Bacterial; Microbial Sensitivity Tests; Molecular Sequence Data; Mutation; mutations; Plant proteins; Rifampin; rpoB gene; Sequence Homology, Amino Acid
Subjects: Q Science > QR Microbiology
NAU Depositing Author Academic Status: Faculty/Staff
Department/Unit: College of Engineering, Forestry, and Natural Science > Biological Sciences
Date Deposited: 30 Sep 2015 05:40
URI: http://openknowledge.nau.edu/id/eprint/342

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