Radnedge, Lyndsay and Agron, Peter G. and Hill, Karen K. and Jackson, Paul J. and Ticknor, Lawrence O. and Keim, Paul and Andersen, Gary L. (2003) Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Applied and Environmental Microbiology, 69 (5). pp. 2755-2764. ISSN 1098-5336
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Abstract
The three species of the group 1 bacilli, Bacillus anthracis, B. cereus, and B. thuringiensis, are genetically very closely related. All inhabit soil habitats but exhibit different phenotypes. B. anthracis is the causative agent of anthrax and is phylogenetically monomorphic, while B. cereus and B. thuringiensis are genetically more diverse. An amplified fragment length polymorphism analysis described here demonstrates genetic diversity among a collection of non-anthrax-causing Bacillus species, some of which show significant similarity to B. anthracis. Suppression subtractive hybridization was then used to characterize the genomic differences that distinguish three of the non-anthrax-causing bacilli from B. anthracis Ames. Ninety-three DNA sequences that were present in B. anthracis but absent from the non-anthrax-causing Bacillus genomes were isolated. Furthermore, 28 of these sequences were not found in a collection of 10 non-anthrax-causing Bacillus species but were present in all members of a representative collection of B. anthracis strains. These sequences map to distinct loci on the B. anthracis genome and can be assayed simultaneously in multiplex PCR assays for rapid and highly specific DNA-based detection of B. anthracis.
| Item Type: | Article |
|---|---|
| ID number or DOI: | 10.1128/AEM.69.5.2755-2764.2003 |
| Keywords: | bacteria; diversity; DNA; gene; Genes; identification; Real-time PCR; sequence; s-layer; soils; strains; subtractive hybridization; yersinia-pestis |
| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology |
| NAU Depositing Author Academic Status: | Faculty/Staff |
| Department/Unit: | College of Engineering, Forestry, and Natural Science > Biological Sciences |
| Date Deposited: | 30 Sep 2015 16:20 |
| URI: | http://openknowledge.nau.edu/id/eprint/350 |
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