About OpenKnowledge@NAU | For NAU Authors

Whole-genome-based phylogeny and divergence of the genus brucella

Foster, Jeffrey T. and Beckstrom-Sternberg, Stephen M and Pearson, Talima Ross and Beckstrom-Sternberg, James S and Chain, Patrick S. G. and Roberto, Francisco F. and Hnath, Jonathan and Brettin, Tom and Keim, Paul (2009) Whole-genome-based phylogeny and divergence of the genus brucella. Journal of Bacteriology, 191 (8). pp. 2864-2870. ISSN 1098-5530

[img]
Preview
Text
Foster_J_etal_2009_Whole-genome-based_phylogeny(1).pdf

Download (176kB) | Preview
Publisher’s or external URL: http://dx.doi.org/10.1128/jb.01581-08

Abstract

Brucellae are worldwide bacterial pathogens of livestock and wildlife, but phylogenetic reconstructions have been challenging due to limited genetic diversity. We assessed the taxonomic and evolutionary relationships of five Brucella species—Brucella abortus, B. melitensis, B. suis, B. canis, and B. ovis—using whole-genome comparisons. We developed a phylogeny using single nucleotide polymorphisms (SNPs) from 13 genomes and rooted the tree using the closely related soil bacterium and opportunistic human pathogen, Ochrobactrum anthropi. Whole-genome sequencing and a SNP-based approach provided the requisite level of genetic detail to resolve species in the highly conserved brucellae. Comparisons among the Brucella genomes revealed 20,154 orthologous SNPs that were shared in all genomes. Rooting with Ochrobactrum anthropi reveals that the B. ovis lineage is basal to the rest of the Brucella lineage. We found that B. suis is a highly divergent clade with extensive intraspecific genetic diversity. Furthermore, B. suis was determined to be paraphyletic in our analyses, only forming a monophyletic clade when the B. canis genome was included. Using a molecular clock with these data suggests that most Brucella species diverged from their common B. ovis ancestor in the past 86,000 to 296,000 years, which precedes the domestication of their livestock hosts. Detailed knowledge of the Brucella phylogeny will lead to an improved understanding of the ecology, evolutionary history, and host relationships for this genus and can be used for determining appropriate genotyping approaches for rapid detection and diagnostic assays for molecular epidemiological and clinical studies.

Item Type: Article
ID number or DOI: 10.1128/JB.01581-08
Keywords: abortus; Bacterial evolution; Biology; Biology--Microbiology; Brucella; Canis; cluster analysis; data processing; DNA, Bacterial; dna polymorphism; Domestic animals; Domestication; Environmental sciences; Evolution; Evolution (Biology); Evolution, Molecular; genetic diversity; Genetic polymorphisms; Genome, Bacterial; Genomes; Genomics; Genotyping; Humans; livestock; Medical sciences; Ochrobactrum anthropi; Opportunist infection; Ovis; pathogens; Phylogeny; Polymorphism, Single Nucleotide; rapid identification; Real-time PCR; rooting; sequence; Single-nucleotide polymorphism; single-nucleotide polymorphisms; soil microorganisms; strains; suis; Wildlife
Subjects: Q Science > QR Microbiology
NAU Depositing Author Academic Status: Faculty/Staff
Department/Unit: Research Centers > Center for Microbial Genetics and Genomics
Date Deposited: 12 Oct 2015 22:26
URI: http://openknowledge.nau.edu/id/eprint/803

Actions (login required)

IR Staff Record View IR Staff Record View

Downloads

Downloads per month over past year