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A horizontal gene transfer event defines two distinct groups within Burkholderia pseudomallei that have dissimilar geographic distributions

Tuanyok, Apichai and Auerbach, Raymond K. and Brettin, Thomas S. and Bruce, David C. and Munk, A. Christine and Detter, J. Chris and Pearson, Talima and Hornstra, Heidie and Sermswan, Rasana W. and Wuthiekanun, Vanaporn and Peacock, Sharon J. and Currie, Bart J. and Keim, Paul and Wagner, David M. (2007) A horizontal gene transfer event defines two distinct groups within Burkholderia pseudomallei that have dissimilar geographic distributions. Journal of Bacteriology, 189 (24). pp. 9044-9049. ISSN 1098-5530

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Publisher’s or external URL: http://dx.doi.org/10.1128/jb.01264-07

Abstract

Burkholderia pseudomallei is the etiologic agent of melioidosis. Many disease manifestations are associated with melioidosis, and the mechanisms causing this variation are unknown; genomic differences among strains offer one explanation. We compared the genome sequences of two strains of B. pseudomallei: the original reference strain K96243 from Thailand and strain MSHR305 from Australia. We identified a variable homologous region between the two strains. This region was previously identified in comparisons of the genome of B. pseudomallei strain K96243 with the genome of strain E264 from the closely related B. thailandensis. In that comparison, K96243 was shown to possess a horizontally acquired Yersinia-like fimbrial (YLF) gene cluster. Here, we show that the homologous genomic region in B. pseudomallei strain 305 is similar to that previously identified in B. thailandensis strain E264. We have named this region in B. pseudomallei strain 305 the B. thailandensis-like flagellum and chemotaxis (BTFC) gene cluster. We screened for these different genomic components across additional genome sequences and 571 B. pseudomallei DNA extracts obtained from regions of endemicity. These alternate genomic states define two distinct groups within B. pseudomallei: all strains contained either the BTFC gene cluster (group BTFC) or the YLF gene cluster (group YLF). These two groups have distinct geographic distributions: group BTFC is dominant in Australia, and group YLF is dominant in Thailand and elsewhere. In addition, clinical isolates are more likely to belong to group YLF, whereas environmental isolates are more likely to belong to group BTFC. These groups should be further characterized in an animal model.

Item Type: Article
Publisher’s Statement: Copyright © 2007, American Society for Microbiology.
ID number or DOI: 10.1128/JB.01264-07
Keywords: animal models; APEC countries; Australasia; Australia; bacteria; bacterium; Betaproteobacteria; Biochemistry; Burkholderia; Burkholderiaceae; Burkholderiales; Burkholderia pseudomallei; causative agent; Chemotaxis; Chromosomes, Bacterial; clinical isolates; Commonwealth of Nations; Developed Countries; DNA; DNA, Bacterial; Environmental Microbiology; epidemiology; Evolution, Molecular; Flagella; Gene clusters; General Molecular Biology (ZZ360) (Discontinued March 2000); Genes; Genetics and Molecular Biology of Microorganisms (ZZ395) (New March 2000); gene transfer; Gene Transfer, Horizontal; genome; Genome analysis; Genomes; Genomics; Genotype; geographical distribution; Gram negative bacteria; human diseases; Humans; identification; mallei; Melioidosis; Molecular epidemiology; Molecular Sequence Data; Multigene Family; northern australia; Nucleotide sequence; Oceania; OECD Countries; polymerase-chain-reaction; Prion, Viral, Bacterial and Fungal Pathogens of Humans (VV210) (New March 2000); Prokaryotes; Proteobacteria; reveals; Sequence Analysis, DNA; Sequence Homology; strain; Synteny; TBZ; Thailand; thailandensis; thiabendazole; tiabendazole
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
NAU Depositing Author Academic Status: Faculty/Staff
Department/Unit: College of Engineering, Forestry, and Natural Science > Biological Sciences
Date Deposited: 20 Oct 2015 22:11
URI: http://openknowledge.nau.edu/id/eprint/817

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