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Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

Kaestli, Mirjam and Richardson, Leisha J. and Colman, Rebecca E. and Tuanyok, Apichai and Price, Erin P. and Bowers, Jolene R. and Mayo, Mark and Kelley, Erin and Seymour, Meagan L. and Sarovich, Derek S. and Pearson, Talima and Engelthaler, David M. and Wagner, David M. and Keim, Paul S. and Schupp, James M. and Currie, Bart J. (2012) Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens. Journal of Clinical Microbiology, 50 (6). pp. 2059-2062. ISSN 1098-660X

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Publisher’s or external URL: http://dx.doi.org/10.1128/jcm.06737-11

Abstract

Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

Item Type: Article
Publisher’s Statement: Copyright © 2012, American Society for Microbiology. All Rights Reserved.
ID number or DOI: 10.1128/JCM.06737-11
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
NAU Depositing Author Academic Status: Faculty/Staff
Department/Unit: Research Centers > Center for Microbial Genetics and Genomics
Date Deposited: 08 Mar 2016 22:24
URI: http://openknowledge.nau.edu/id/eprint/837

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