High frequency, spontaneous motA mutations in Campylobacter jejuni strain 81-176

Mohawk, Krystle L. and Poly, Frédéric and Sahl, Jason W. and Rasko, David A. and Guerry, Patricia (2014) High frequency, spontaneous motA mutations in Campylobacter jejuni strain 81-176. PLoS ONE, 9 (2). e88043. ISSN 1932-6203

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Abstract

Campylobacter jejuni is an important cause of bacterial diarrhea worldwide. The pathogenesis of C. jejuni is poorly understood and complicated by phase variation of multiple surface structures including lipooligosaccharide, capsule, and flagellum. When C. jejuni strain 81-176 was plated on blood agar for single colonies, the presence of translucent, non-motile colonial variants was noted among the majority of opaque, motile colonies. High-throughput genomic sequencing of two flagellated translucent and two opaque variants as well as the parent strain revealed multiple genetic changes compared to the published genome. However, the only mutated open reading frame common between the two translucent variants and absent from the opaque variants and the parent was motA, encoding a flagellar motor protein. A total of 18 spontaneous motA mutations were found that mapped to four distinct sites in the gene, with only one class of mutation present in a phase variable region. This study exemplifies the mutative/adaptive properties of C. jejuni and demonstrates additional variability in C. jejuni beyond phase variation.

Item Type:	Article
ID number or DOI:	10.1371/journal.pone.0088043
Keywords:	Campylobacter jejuni; bacterial diarrhea; pathogenesis; phase variation; colonies; genomic sequencing; foodborne illness;
Subjects:	Q Science > QH Natural history > QH301 Biology
NAU Depositing Author Academic Status:	Faculty/Staff
Department/Unit:	College of Engineering, Forestry, and Natural Science > Biological Sciences
Date Deposited:	16 Oct 2015 21:16
URI:	http://openknowledge.nau.edu/id/eprint/1707

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