Molecular investigations of a locally acquired case of melioidosis in southern AZ, USA.

Engelthaler, David M. and Bowers, Jolene and Schupp, James A. and Pearson, Talima and Ginther, Jennifer and Hornstra, Heidie M. and Dale, Julia and Stewart, Tasha and Sunenshine, Rebecca and Waddell, Victor and Levy, Craig and Gillece, John and Price, Lance B. and Contente, Tania and Beckstrom-Sternberg, Stephen M. and Blaney, David D. and Wagner, David M. and Mayo, Mark and Currie, Bart J. and Keim, Paul and Tuanyok, Apichai (2011) Molecular investigations of a locally acquired case of melioidosis in southern AZ, USA. PLoS Neglected Tropical Diseases, 5 (10). e1347. ISSN 1935-2727

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Abstract

Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and no previous history of disease. Diagnosis of the case was confirmed through multiple microbiologic and molecular techniques. To enhance the epidemiological analysis, we conducted several molecular genotyping procedures, including multi-locus sequence typing, SNP-profiling, and whole genome sequence typing. Each technique has different molecular epidemiologic advantages, all of which provided evidence that the infecting strain was most similar to those found in Southeast Asia, possibly originating in, or around, Malaysia. Advancements in new typing technologies provide genotyping resolution not previously available to public health investigators, allowing for more accurate source identification.

Item Type:	Article
Publisher’s Statement:	Creative Commons Attribution License.
ID number or DOI:	10.1371/journal.pntd.0001347.t001
Keywords:	molecular genotyping; Arizona epidemiology; cluster analysis; molecular epidemology; burkholderia pseudomallei;
Subjects:	Q Science > QR Microbiology
NAU Depositing Author Academic Status:	Faculty/Staff
Department/Unit:	College of Engineering, Forestry, and Natural Science > Biological Sciences
Date Deposited:	07 Oct 2015 19:33
URI:	http://openknowledge.nau.edu/id/eprint/694

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