Shuey, Megan M. and Drees, Kevin P. and Lindner, Daniel L. and Keim, Paul S and Foster, Jeffrey T. (2014) Highly sensitive quantitative PCR for the detection and differentiation of Pseudogymnoascus destructans and other Pseudogymnoascus species. Applied & Environmental Microbiology, 80 (5). pp. 1726-1731. ISSN 1098-5336
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Abstract
White-nose syndrome is a fungal disease that has decimated bat populations across eastern North America. Identification of the etiologic agent, Pseudogymnoascus destructans (formerly Geomyces destructans), in environmental samples is essential to proposed management plans. A major challenge is the presence of closely related species, which are ubiquitous in many soils and cave sediments and often present in high abundance. We present a dual-probe real-time quantitative PCR assay capable of detecting and differentiating P. destructans from closely related fungi in environmental samples from North America. The assay, based on a single nucleotide polymorphism (SNP) specific to P. destructans, is capable of rapid low-level detection from various sampling media, including sediment, fecal samples, wing biopsy specimens, and skin swabs. This method is a highly sensitive, high-throughput method for identifying P. destructans, other Pseudogymnoascus spp., and Geomyces spp. in the environment, providing a fundamental component of research and risk assessment for addressing this disease, as well as other ecological and mycological work on related fungi.
| Item Type: | Article |
|---|---|
| ID number or DOI: | 10.1128/AEM.02897-13 |
| Keywords: | Ascomycota; bat hibernacula; bats; Cell differentiation; DNA, Fungal; Environmental Microbiology; fungal pathogen; Geomyces destructans; geomyces-destructans; Infection; Mycology; Mycoses; North America; Oligonucleotide Probes; pannorum; Polymerase chain reaction; Polymorphism, Single Nucleotide; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity; Single nucleotide polymorphisms; soils; White-nose syndrome |
| Subjects: | Q Science > QR Microbiology |
| NAU Depositing Author Academic Status: | Faculty/Staff |
| Department/Unit: | Research Centers > Center for Microbial Genetics and Genomics |
| Date Deposited: | 30 Sep 2015 05:49 |
| URI: | http://openknowledge.nau.edu/id/eprint/344 |
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