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Dual-Projection Digital Holographic Tomography

Bradfield, Andrew (2021) Dual-Projection Digital Holographic Tomography. Masters thesis, Northern Arizona University.

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Digital Holographic Tomography (DHT) was applied to the microscopic imaging of biological samples. This technique creates images that retain information about the sample’s features that is lost with optical microscopy techniques, such as its physical height or its index of refraction. DHT creates two-dimensional slices through a sample at different depths, allowing for the study of internal features that can be missed with an optical microscope. The slices are intentionally created using a set of scanning mirrors which sweep across the sample. The addition of a second mirror allows for the sample to be scanned in the X direction and Y direction. The experiment is set up within a commercial microscope, allowing the system to sit in a vertical configuration. This allows the sample to rest easily on an X-Y plane granting easier analysis without manipulating the sample in any way. Slices at different heights within each sample have been generated using MATLAB and stacked to recreate a three-dimensional figure of the sample. The addition of the second mirror allows for a more complete reconstruction of the sample at each height with the additional data. This technique has proven to be excellent for studying transparent samples with no manipulation.

Item Type: Thesis (Masters)
Publisher’s Statement: © Copyright is held by the author. Digital access to this material is made possible by the Cline Library, Northern Arizona University. Further transmission, reproduction or presentation of protected items is prohibited except with permission of the author.
Keywords: Holography; Microscopy; Tomography
Subjects: Q Science > QC Physics
NAU Depositing Author Academic Status: Student
Department/Unit: Graduate College > Theses and Dissertations
College of the Environment, Forestry, and Natural Sciences > Physics and Astronomy
Date Deposited: 07 Feb 2022 16:52
Last Modified: 07 Feb 2022 16:52
URI: https://openknowledge.nau.edu/id/eprint/5667

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