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Polymer encapsulated DNA vaccine for sustained release

Pedersen, Christine (2021) Polymer encapsulated DNA vaccine for sustained release. Masters thesis, Northern Arizona University.

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To improve the efficacy of single-dose vaccines, we have developed stabilizer-free poly lactic-co-glycolic acid (PLGA) nanoparticle formulations for use as drug and vaccine delivery systems capable of sustained-release. By removing commonly used toxic stabilizers such as poly vinyl alcohol (PVA), we were able to increase cellular viability, while maintaining stability of PLGA nanoparticles through optimization of PLGA composition. Nanoparticle stability is important to shelf life, vaccine encapsulation, and extended release for persistent immune activation. By altering charge, formulation temperature and protein addition, we have developed PLGA nanoparticles with tunable degradation over a one-to-two-month time periods. These improvements in nanoparticle delivery systems should provide for increased efficacy of single-dose vaccines by mimicking the prime-boost schedule currently used.

Item Type: Thesis (Masters)
Publisher’s Statement: © Copyright is held by the author. Digital access to this material is made possible by the Cline Library, Northern Arizona University. Further transmission, reproduction or presentation of protected items is prohibited except with permission of the author.
Keywords: Polylactic-co-glycolic acid; vaccine delivery systems; drug delivery systems; PLGS nanoparticles;
Subjects: Q Science > QD Chemistry
NAU Depositing Author Academic Status: Student
Department/Unit: Graduate College > Theses and Dissertations
College of Engineering, Informatics, and Applied Sciences > Civil Engineering, Construction Management and Environmental Engineering
Date Deposited: 11 Feb 2022 21:15
Last Modified: 11 Feb 2022 21:15
URI: https://openknowledge.nau.edu/id/eprint/5689

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