Multiple-Wavelength Phase Retrieval With Digital Holographic Microscopy

Ayisi, Obed Aboah (2021) Multiple-Wavelength Phase Retrieval With Digital Holographic Microscopy. Masters thesis, Northern Arizona University.

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Abstract

The phase of an image is very important because it is the phase, not the amplitude, that carries most of the information in an image. A traditional bright-field microscope only captures the amplitude information from a sample, and are therefore not of great use in applications where the sample is partially or totally transparent. Digital Holographic Microscopy (DHM) provides a phase retrieval technique that can obtain both the amplitude and phase of a sample. In this thesis, an iterative phase retrieval method for DHM using two illumination wavelengths is proposed which exhibits high immunity to noise and yields wrap-free phase images of biological samples when the image is reconstructed. Experimental results of human cheek cells, human blood smear, and neurons are presented to demonstrate the approach.

Item Type:	Thesis (Masters)
Publisher’s Statement:	© Copyright is held by the author. Digital access to this material is made possible by the Cline Library, Northern Arizona University. Further transmission, reproduction or presentation of protected items is prohibited except with permission of the author.
Keywords:	Digital Holographic Microscopy; Phase retrieval; Phase Unwrapping
Subjects:	Q Science > QC Physics
NAU Depositing Author Academic Status:	Student
Department/Unit:	Graduate College > Theses and Dissertations College of the Environment, Forestry, and Natural Sciences > Physics and Astronomy
Date Deposited:	18 Feb 2022 16:46
Last Modified:	18 Feb 2022 16:46
URI:	https://openknowledge.nau.edu/id/eprint/5717

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