Antimicrobial activity of novel ionic liquids/deep eutectic solvents help to resist biofouling

Nguyen, Marjorie AnhTu (2022) Antimicrobial activity of novel ionic liquids/deep eutectic solvents help to resist biofouling. Masters thesis, Northern Arizona University.

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Abstract

Most nosocomial infections, or patient carrying diseases, arise from antibiotic-resistant pathogens, and these organisms can create a number of medically relevant concerns. Pathogenic organisms can impact wound healing, tissue transportation, or medical grade equipment used in clinical settings and around at-risk patient populations. An example of surface contamination in wound healing wound be diabetic patients whose wounds are unmanaged are at risk of amputation due to the progression of nonhealing diabetic ulcers that are contaminated by antibiotic resistant pathogens in the wound beds. New methods to address these microbial contaminations are needed. In the tissue transplantation industry, pathogen surveillance is necessary to ensure procedures remain aseptic post-harvest, during transportation, and up to the point of transplantation to the recipient. Decontamination of medical equipment or medical devices such as urinary catheters are another space where pathogen contamination can present compounding complications. Clean medical grade equipment, such as medical catheter tubing, also pose a risk of transporting external microbes to internal sensitive tissues. Taking advantage of known antimicrobial solutions that would manage infected tissues used for transplantation, such as ionic liquids/deep eutectic solvents (IL/DES), would prove beneficial for the tissue transplantation process. IL/DES have been developed as antiseptic agents with antimicrobial properties used for disrupting the structure of biofilms in antibiotic resistant microbes. In this study, antimicrobial IL/DES were characterized using a dose-response assessment to determine cellular compatibility and antimicrobial properties. The PrestoBlue and CyQUANT assays were used in growth media for adult human dermal fibroblasts cells to determine biocompatibility and in microbroth dilution antibiotic assays for antibiotic resistant microbes to determine the antimicrobial properties. Current results demonstrated promising cellular compatibility when using low doses of IL/DES on human dermal fibroblast cells. The antimicrobial activity results depict the activity is present in the novel IL/DES and retained when pre-treating medical grade equipment to reduce or eliminate antibiotic resistant infections.

Item Type:	Thesis (Masters)
Publisher’s Statement:	© Copyright is held by the author. Digital access to this material is made possible by the Cline Library, Northern Arizona University. Further transmission, reproduction or presentation of protected items is prohibited except with permission of the author.
Keywords:	antibiotic resistance; antiseptic; catheter device; ionic liquids/ deep eutectic solvents; tissue transplantation; wound healing
Subjects:	R Medicine > RB Pathology
MeSH Subjects:	C Diseases > C01 Bacterial Infections and Mycoses
NAU Depositing Author Academic Status:	Student
Department/Unit:	Graduate College > Theses and Dissertations College of the Environment, Forestry, and Natural Sciences > Biological Sciences
Date Deposited:	26 May 2023 16:46
Last Modified:	26 May 2023 16:46
URI:	https://openknowledge.nau.edu/id/eprint/5912

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