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Francisella tularensis strain typing using multiple-locus, variable-number tandem repeat analysis

Farlow, Jason and Smith, Kimothy L. and Wong, Jane and Abrams, Michelle and Lytle, Michael and Keim, Paul (2001) Francisella tularensis strain typing using multiple-locus, variable-number tandem repeat analysis. Journal of Clinical Microbiology, 39 (9). pp. 3186-3192. ISSN 1098-660X


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Publisher’s or external URL: http://dx.doi.org/10.1128/JCM.39.9.3186-3192.2001


Francisella tularensis, the etiological agent of tularemia, is found throughout the Northern hemisphere. After analyzing the F. tularensis genomic sequence for potential variable-number tandem repeats (VNTRs), we developed a multilocus VNTR analysis (MLVA) typing system for this pathogen. Variation was detected at six VNTR loci in a set of 56 isolates from California, Oklahoma, Arizona, and Oregon and the F. tularensis live vaccine strain. PCR assays revealed diversity at these loci with total allele numbers ranging from 2 to 20, and Nei's diversity index values ranging from 0.36 to 0.93. Cluster analysis identified two genetically distinct groups consistent with the current biovar classification system ofF. tularensis. These findings suggest that these VNTR markers are useful for identifying F. tularensisisolates at this taxonomic level. In this study, biovar B isolates were less diverse than those in biovar A, possibly reflecting the history of tularemia in North America. Seven isolates from a recent epizootic in Maricopa County, Ariz., were identical at all VNTR marker loci. Their identity, even at a hypervariable VNTR locus, indicates a common source of infection. This demonstrates the applicability of MLVA for rapid characterization and identification of outbreak isolates. Future construction of reference databases will allow faster outbreak tracking as well as providing a foundation for deciphering global genetic relationships.

Item Type: Article
Publisher’s Statement: Copyright © 2001, American Society for Microbiology
ID number or DOI: 10.1128/JCM.39.9.3186-3192.2001
Keywords: Bacterial Typing Techniques/methods; Francisella tularensis/classification; Francisella tularensis; Minisatellite Repeats/genetics; Polymerase Chain Reaction; Tularemia/microbiology
Subjects: Q Science > QR Microbiology
NAU Depositing Author Academic Status: Faculty/Staff
Department/Unit: College of Engineering, Forestry, and Natural Science > Biological Sciences
Date Deposited: 29 Jan 2016 22:21
URI: http://openknowledge.nau.edu/id/eprint/833

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