Reciprocal interactions between the bark beetle-associated yeast Ogataea pini and host plant phytochemistry

Davis, Thomas S. and Hofstetter, Richard W. (2011) Reciprocal interactions between the bark beetle-associated yeast Ogataea pini and host plant phytochemistry. Mycologia, 103 (6). pp. 1201-1207. ISSN 1557-2536

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Abstract

Here we report the first experiments testing reciprocal effects between the bark beetle-associated yeast, Ogataea pini, and phytochemicals present in tree tissues (Pinus ponderosa). We tested two hypotheses: (i) tree phytochemicals mediate O. pin,i growth and (ii) O. pini affects chemical composition of plant tissues. We tested six monoterpenes on O. pins biomass growth in vitro and found that most monoterpenes inhibited O. pini growth; however mean O. pini biomass increased 21.5% when treated with myrcene and 75.5% when treated with terpinolene, relative to control. Ogataea pini was grown on phloem tissue ex vivo to determine whether O. pini affected phloem chemistry. Monoterpene concentrations declined in phloem over time, but phloem colonized by O. pini had significantly different concentrations of monoterpenes at two periods than phloem with no yeast. After 7 d, when O. pini was present, concentrations of the monoterpene Delta-3-carene was 42.9% lower than uncolonized phloem and concentrations of the monoterpene terpinolene was 345.0% higher than uncolonized phloem. After 15 d phloem colonized by O. pini had 505.4% higher concentrations of a-pinene than uncolonized phloem. These experiments suggest that O. pini responds to phytochemicals present in host tissues and the presence of O. pini might alter the chemical environment of phloem tissues during the early stages of beetle development. The interactions between O. pini and phytochemicals in pine vascular tissues might have consequences for the bark beetle that vectors O. pini, Dendroctonus brevicomis.

Item Type:	Article
Publisher’s Statement:	Copyright 2011 by The Mycological Society of America
ID number or DOI:	10.3852/11-083
Keywords:	Animals; bark beetle; Beetles; Behavior; biomass; Coleoptera; Dendroctonus brevicomis; dendroctonus-ponderosae; Fungi; growth; Index Medicus; monoterpene; monoterpenes; Ogataea pini; Oleoresin; Phytochemistry; Pinus; Pinus Ponderosa; Saccharomycetales; Scolytidae; symbiosis; yeast
Subjects:	Q Science > QH Natural history > QH301 Biology S Agriculture > SD Forestry
NAU Depositing Author Academic Status:	Faculty/Staff
Department/Unit:	College of Engineering, Forestry, and Natural Science > School of Forestry
Date Deposited:	16 Oct 2015 17:21
URI:	http://openknowledge.nau.edu/id/eprint/1032

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