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Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

Easterday, W. Ryan and Van Ert, Matthew N. and Simonson, Tatum S. and Wagner, David M. and Kenefic, Leo J. and Allender, Christopher J. and Keim, Paul (2005) Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis. Journal of Clinical Microbiology, 43 (4). pp. 1995-1997. ISSN 1098-660X

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Publisher’s or external URL: http://dx.doi.org/10.1128/jcm.43.4.1995-1997.2005

Abstract

A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

Item Type: Article
Publisher’s Statement: Copyright © 2005, American Society for Microbiology.
ID number or DOI: 10.1128/JCM.43.4.1995-1997.2005
Keywords: real time PCR Bacillus species identification; sequence Bacillus gene plcR transcription factor SNP
Subjects: Q Science > QR Microbiology
MeSH Subjects: B Organisms > B03 Bacteria
NAU Depositing Author Academic Status: Faculty/Staff
Department/Unit: College of Engineering, Forestry, and Natural Science > Biological Sciences
Date Deposited: 29 Jan 2016 22:13
URI: http://openknowledge.nau.edu/id/eprint/830

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