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A North American Yersinia pestis draft genome sequence: SNPs and phylogenetic analysis

Touchman, Jeffrey W. and Wagner, David M. and Hao, Jicheng and Mastrian, Stephen D. and Shah, Maulik K. and Vogler, Amy J. and Allender, Christopher J. and Clark, Erin A. and Benitez, Debbie S. and Youngkin, David J. and Girard, Jessica M. and Auerbach, Raymond K. and Beckstrom-Sternberg, Stephen M. and Keim, Paul (2007) A North American Yersinia pestis draft genome sequence: SNPs and phylogenetic analysis. PLoS ONE, 2 (2). e220. ISSN 1932-6203

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Publisher’s or external URL: http://dx.doi.org/10.1371/journal.pone.0000220


Background. Yersinia pestis, the causative agent of plague, is responsible for some of the greatest epidemic scourges of mankind. It is widespread in the western United States, although it has only been present there for just over 100 years. As a result, there has been very little time for diversity to accumulate in this region. Much of the diversity that has been detected among North American isolates is at loci that mutate too quickly to accurately reconstruct large-scale phylogenetic patterns. Slowly-evolving but stable markers such as SNPs could be useful for this purpose, but are difficult to identify due to the monomorphic nature of North American isolates.Methodology/Principal FindingsTo identify SNPs that are polymorphic among North American populations of Y. pestis, a gapped genome sequence of Y. pestis strain FV-1 was generated. Sequence comparison of FV-1 with another North American strain, CO92, identified 19 new SNP loci that differ among North American isolates.Conclusions/SignificanceThe 19 SNP loci identified in this study should facilitate additional studies of the genetic population structure of Y. pestis across North America.

Item Type: Article
Publisher’s Statement: Specific CC license required, no statement seen
ID number or DOI: 10.1371/journal.pone.0000220
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Keywords: Yersinia pestis; Y. pestis; genome sequence; plague; FV-1; CO92;
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
NAU Depositing Author Academic Status: Faculty/Staff
Department/Unit: College of Engineering, Forestry, and Natural Science > Biological Sciences
Research Centers > Center for Microbial Genetics and Genomics
Date Deposited: 16 Oct 2015 17:50
URI: http://openknowledge.nau.edu/id/eprint/1694

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