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Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors

Liguori, Andrew P. and Warrington, Stephanie D. and Ginther, Jennifer L. and Pearson, Talima and Bowers, Jolene and Glass, Mindy B. and Mayo, Mark and Wuthiekanun, Vanaporn and Engelthaler, David and Peacock, Sharon J. and Currie, Bart J. and Wagner, David M. and Keim, Paul S and Tuanyok, Apichai (2011) Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors. PLoS ONE, 6 (12). e29323. ISSN 1932-6203

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Publisher’s or external URL: http://dx.doi.org/10.1371/journal.pone.0029323


Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near-relative species. B. pseudomallei is known as one of the most genetically recombined bacterial species. In silico analysis of multiple B. pseudomallei genomes revealed approximately four homologous rRNA operons and ITS length polymorphisms therein. We characterized ITS distribution using PCR and analyzed via a high-throughput capillary electrophoresis in 1,191 B. pseudomallei strains. Three major ITS types were identified, two of which were commonly found in most B. pseudomallei strains from the endemic areas, whereas the third one was significantly correlated with worldwide sporadic strains. Interestingly, mixtures of the two common ITS types were observed within the same strains, and at a greater incidence in Thailand than Australia suggesting that genetic recombination causes the ITS variation within species, with greater recombination frequency in Thailand. In addition, the B. mallei ITS type was common to B. pseudomallei, providing further support that B. mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species.

Item Type: Article
ID number or DOI: 10.1371/journal.pone.0029323
Keywords: Base Sequence; Burkholderia pseudomallei; Computational biology; DNA, Bacterial; DNA, Ribosomal; DNA, Ribosomal Spacer; epidemiology; Evolution, Molecular; Gel electrophoresis; gene; Genetic markers; Genetic polymorphisms; Genetic recombination; Genetic transcription; Genetic variation; Genome, Bacterial; Geography; identification; mallei; Meliodoisis; Molecular Sequence Data; Operon; operon copy number; Phylogeny; Polymerase chain reaction; pseudomonas-pseudomallei; Recombinant DNA; region; Ribosomal DNA; sequence-analysis; Sequence Analysis, DNA; soil
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
NAU Depositing Author Academic Status: Faculty/Staff
Department/Unit: College of Engineering, Forestry, and Natural Science > Biological Sciences
Research Centers > Center for Microbial Genetics and Genomics
Date Deposited: 16 Oct 2015 18:16
URI: http://openknowledge.nau.edu/id/eprint/1712

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