Easterday, W. Ryan and Van Ert, Matthew N. and Simonson, Tatum S. and Wagner, David M. and Kenefic, Leo J. and Allender, Christopher J. and Keim, Paul (2005) Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis. Journal of Clinical Microbiology, 43 (4). pp. 1995-1997. ISSN 1098-660X
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Abstract
A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.
Item Type: | Article |
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Publisher’s Statement: | Copyright © 2005, American Society for Microbiology. |
ID number or DOI: | 10.1128/JCM.43.4.1995-1997.2005 |
Keywords: | real time PCR Bacillus species identification; sequence Bacillus gene plcR transcription factor SNP |
Subjects: | Q Science > QR Microbiology |
MeSH Subjects: | B Organisms > B03 Bacteria |
NAU Depositing Author Academic Status: | Faculty/Staff |
Department/Unit: | College of Engineering, Forestry, and Natural Science > Biological Sciences |
Date Deposited: | 29 Jan 2016 22:13 |
URI: | http://openknowledge.nau.edu/id/eprint/830 |
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